FIGURE 4.

Pharmacologic inhibition of EZH2 reduces global H3K27me3 levels in vitro. A, immunoblot analysis of EZH2 and H3K27me3 levels in CD4⁺CD25⁺ cells cultured in the presence of the indicated inhibitors for 18 h. B, immunoblot analysis of EZH2 and H3K27me3 levels in CD4⁺CD62L⁺ cells cultured under Treg-stimulating conditions (see “Experimental Procedures”) in the presence of the indicated inhibitors for 36 h. Note the toxicity of GSK126 and EPZ4638, as evident by reduction of Ponceau staining under these conditions.