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. 2016 Dec 1;292(2):706–722. doi: 10.1074/jbc.M116.749663

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FIGURE 6. — Pharmacologic inhibition of EZH2 reduces global H3K27me3 levels in vivo. A, representative immunoblot analysis of EZH2 and H3K27me3 levels in CD4⁺CD25⁺ cells, colon, and pancreas harvested from DZNep + 2% DSS-treated animals. B, quantification by densitometry of H3K27me3 immunoblot bands from isolated Treg cells in four independent experiments. For densitometry analysis, significance was determined using a non-parametric, unpaired t test of significance (Mann-Whitney). *, p < 0.05. The data are generated from n = 8 animals, 4/group.