FIGURE 2.

(−)-EA-activated Hs578T channels are not TRPC4 homomers. A, (−)-EA current in Hs578T. A whole-cell voltage clamp recording of membrane current during ramp changes in membrane voltage from −100 to +100 mV applied every 10 s is shown. Only currents sampled at −100 and +100 mV are displayed. 100 nm (−)-EA was bath-applied as indicated by the horizontal bar. B, full current traces during two ramp changes in voltage, one during the initial application of vehicle (DMSO) and the other after application of (−)-EA. Note the absence of a seatlike inflection characteristic of TRPC4 currents (24).