FIGURE 9.
(−)-EA-evoked Tl+ entry in A498 and Hs578T cells is Ca2+-dependent. A and B, example of 96-well plate FluxOR measurements of the change (Δ) in the intracellular Tl+ concentration upon application of extracellular Tl+ and 300 nm (−)-EA. Responses were observed in the presence of 0 or 1.5 mm extracellular Ca2+ ([Ca2+]ex) (n = 4). Error bars indicate S.D. C and D, as for A and B but in the continuous presence of 1.8 mm extracellular Ca2+ plus 0 or 2 mm EGTA to buffer Ca2+ (n = 4). E and F, summary data for experiments of the type shown in A–D in which the rate of change of F was measured between 5 and 35 s after (−)-EA application. Each data point represents a value from an independent experiment with mean values indicated alongside (in E, N = 3; in F, N = 4). Percentages indicate decreases in the mean compared with the respective controls. Error bars represent S.D.