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. 2017 Jan 18;7:40498. doi: 10.1038/srep40498

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Samples for Western blot (lanes 1 and 2) and SDS-PAGE analysis (lanes 3 to 5) were as follows: concentrated supernatant from Escherichia coli with the empty vector pET-30a (lanes 2 and 3; control); concentrated supernatant from E. coli with the recombinant vector NlSEF1:pET-30a (lanes 1 and 4); purified recombinant protein NlSEF1 (lane 5) and protein marker (M). Rabbit anti-NlSEF1 polyclonal antibodies were used to develop Western blot.