Figure 1. miR-497-5p is upregulated in the myofibroblast differentiation of lung resident mesenchymal stem cells (LR-MSCs) and the lung tissues from a mouse pulmonary fibrosis model.

(A) A heat map represents the differentially expressed miRNAs in TGF-β1-treated LR-MSCs at day 7. The upregulated miRNAs are indicated in progressively brighter shades of red, and the down-regulated miRNAs are indicated in progressively darker shades of black. (B) Quantitative real-rime polymerase chain reaction analysis validation of the microarray results from LR-MSCs administered with TGF-β1 for 7 consecutive days. The data are expressed as the mean ± SD. *P < 0.05, **P < 0.01. (C) The mice were injected intratracheally with either saline or bleomycin, and sacrificed on day 14 after injection (n = 6 mice in each group). The 11 miRNAs were also investigated in a bleomycin-induced pulmonary fibrosis model as determined by the quantitative real-rime polymerase chain reaction analysis.