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. 2017 Jan 18;7:40958. doi: 10.1038/srep40958

Figure 2. Reck is downregulated in vitro and in vivo as a target gene of miR-497-5p.

Figure 2

(A) Lung resident mesenchymal stem cells (LR-MSCs) were incubated with TGF-β1 for 7 days. The level of Reck was determined by Western blot and quantitative real-time polymerase chain reaction. (B) Mice were administered with bleomycin, and the Reck expression of lung tissues was measured by Western blot and quantitative real-rime polymerase chain reaction. (C) The position of the miR-497-5p target site in the Reck 3′-UTR and the sequence alignment of miR-497-5p and the Reck 3′-UTR from various species are shown. (D) LV-miR-497-5p or negative control (LV-NC) was co-transfected with the mouse Reck 3′-UTR-derived GV306 vector, i.e. negative control (NC), wild type (WT-Reck) or mutated type (MUT-Reck) in the putative miR-497-5p seed region, into 293 T cells. The cells were harvested 48 h after transfection, and luciferase activity was analyzed. The values are presented as the means ± SD. The experiments were performed three times with similar results. **P < 0.01.