Figure 10.

Combined treatment with LiCl and VPA potentiates Lef/Tcf-dependent transcriptional activity and nuclear β-catenin protein level in CGCs. A, CGCs were transfected with Lef-OT reporter construct before plating, using an Amaxa Nucleofector. At 6 DIV, CGCs were treated with 3 mm LiCl and/or the indicated concentrations of VPA for 24 h. Cells were then lysed for assay of Lef/Tcf-dependent luciferase activity. Data are means ± SEM of fold increase relative to the empty vector control from three independent experiments. B, CGCs at 6 DIV were treated with 3 mm LiCl, 0.8 mm VPA, or their combination for 24 h. Cells were then harvested, and nuclear proteins were prepared for Western blotting analysis of β-catenin. GAPDH was used as a loading control.