Figure 12.

Effects of HDAC inhibitors in the absence or presence of lithium on histone acetylation. CGCs at 6 DIV were treated with 3 mm LiCl in the absence or presence of 0.8 mm VPA (A), 1 mm SB (B), 1 mm PB (C), or 20 nm TSA (D) for 24 h. Cells were harvested for Western blotting analysis of acetylated histone-H3 (AH3) using antibodies directed against both Lys9 and Lys14 acetylation or only Lys9 or Lys14 acetylation for A and B, and only Lys9 and Lys14 coacetylations were measured for C and D. β-Actin protein levels were used as the loading control.