Fig. 9.

Translocating intestinal bacteria in multidrug resistant P. aeruginosa infected human microbiota-associated mice suffering from acute ileitis. Human microbiota-associated mice were perorally challenged with T. gondii ME49 to induce acute ileitis and either additionally infected with MDR P. aeruginosa 3 days following ileitis induction (PA; filled bars) or not (N; open bars). At day 7 post ileitis induction translocation rates of a intestinal bacterial species and of b P. aeruginosa to extra-intestinal and systemic compartments were determined by cultivation of homogenated ex vivo biopsies derived from mesenteric lymph nodes (MLN), liver, lung and spleen (direct plating on solid media) and of cardiac blood (in thioglycolate enrichment broths) with subsequent subcultivation and species identification. Mean translocation rates (%) ±SD and absolute numbers of positive samples out of total number analyzed (in parentheses) are indicated. Data shown were pooled from three independent experiments