Fig. 5.

Analysis of glucocorticoid responses of distinct GR-binding sites of glucocorticoid-repressed genes. Reporter plasmids (75 ng) were cotransfected with pcDNA3-hGR (150 ng) and RSV-βGal (100 ng) into A549 cells in a 24-well plate. After 4 h, cells were washed with PBS and treated with 0.1 μM dexamethasone for an additional 16–20 h. Cells were then lysed and assayed for luciferase and β-Gal activities. Data represent the SEM of the percentage of full luciferase expression (dexamethasone-treated cells divided by ethanol-treated cells) of luciferase activity from at least three experiments.