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Immunofluorescence analysis of mitochondrial Hsp70. Adipocytes were isolated from the epididymal fat pads of 26-week-old C57BL/6J, ob/ob, and rosiglitazone-treated ob/ob (ob/ob + Rosi) mice. Adipocytes were fixed and stained and image stacks were obtained with a conventional wide-field microscope fitted with a 40× or 100× Nikon plan-apo objective. Images represent single optical sections. White arrows indicate small, droplet-like structures seen only in rosiglitazone-treated cells.
