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Antioxidant activity is higher in brains from CR animals. The activity of antioxidant enzymes was assayed in whole‐brain homogenates (A, B) or mitochondrial fractions (C). (A) Enzymatic activity of glutathione peroxidase (Gpx‐1). (B) Enzymatic activity of glutathione reductase (GR). (C) Enzymatic activity of superoxide dismutase in mitochondrial fractions (SOD mit). Results are shown as mean ± SEM. **P < 0.01; ***P < 0.001 two‐tailed, unpaired Student's t‐test.
