Figure 4.

Serum from CR animals protects against excitotoxicity and increases mitochondrial respiration in vitro. Cerebellar granule neurons were treated for 24 h with either AL (white bars/symbols) or CR serum (black bars/symbols) and their bioenergetic properties studied. Metabolic parameters were determined as indicated in the Experimental procedures section. (A) Cell death after glutamate challenge. (B) Typical experiment measuring oxygen consumption rates (OCR) using the Seahorse XF Analyzer. Drugs were injected at indicated time points. (C) OCR quantification under basal conditions and after glutamate stimulation. (D) Citrate synthase activity. (E) Western blot for Sirt3 using actin as loading control. Results are shown as mean ± SEM. *P < 0.05 two‐tailed, paired Student's t‐test.