Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 13;16(1):73–81. doi: 10.1111/acel.12527

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Calcium retention capacity is increased in brains from CR animals. Calcium uptake in brain mitochondria from AL or CR animals was monitored using Calcium Green‐5N as described in Experimental procedures. (A) Representative traces. Each peak corresponds to a 100 μm calcium addition. (B) Representative traces in the presence of 5 μm cyclosporin A (CsA). (C) Quantification of calcium retention capacity (CRC). (D) Western blot against cyclophilin D (CypD) using Hsp60 as loading control. (E) Peptidyl prolyl isomerase activity from brain AL or CR mitochondrial fractions. (F) Immunoprecipitation of mitochondrial samples from AL and CR brains with anti‐CypD antibody and blotting using anti‐CypD and anti‐acetylated lysine antibodies. The negative control (‐ CypD) was performed without anti‐CypD. Results are shown as mean ± SEM. *P < 0.05; ***P < 0.001 two‐tailed, unpaired Student's t‐test.