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. 2016 Sep 13;16(1):39–51. doi: 10.1111/acel.12523

Figure 2.

Figure 2

Mitochondrial reactive oxygen species (ROS) accumulation levels. (A) Micrographs of mitochondrial ROS‐generated fluorescence detected by the MitoSOX Red chemical probe on in vitro‐cultured hippocampal tissue sections for confirmation of in vivo accumulation levels. White arrows indicate the fluorescent‐positive astrocytes. Scale bar = 100 μm. White squares are described with higher magnification images in Fig. S5B (Supporting information). (B) The statistical bar charts of red fluorescent intensities on the dentate gyrus (DG), CA3, and CA1 regions of young adult (YA) and middle‐aged (MA) mice, respectively. The white and black bars indicate the MitoSOX Red's fluorescent intensities in wild‐type C57BL/6J and Tet‐mev‐1 mice, respectively. Data are expressed as mean ± SD; *P < 0.05; n = 3 in each group.