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. 2016 Sep 13;16(1):39–51. doi: 10.1111/acel.12523

Figure 6.

Figure 6

Basal and inductive phosphorylated CREB levels, long‐term spatial memory abilities using Morris water maze, and the upstream key enzyme activities for CREB phosphorylation. (A,B) Western blot images using phospho‐CREB (Ser133) and CREB antibodies to total protein lysate in the hippocampal area. The statistical bar charts of internal standardized basal (A) and inductive (B) phosphorylated CREB/CREB ratios by β‐actin levels relative to young adult wild‐type C57BL/6J. White and black bars indicate the wild‐type C57BL/6J and Tet‐mev‐1 mice, respectively. All data are expressed as mean ± SD; *P < 0.05; **P < 0.01; n = >7 in each group (A); n = 6 in each age (B). (C) Long‐term spatial memory test was performed after 5‐day learning using Morris water maze. White and black bars indicate the wild‐type C57BL/6J and Tet‐mev‐1 mice, respectively. Data are expressed as mean ± SD; *P < 0.01; **P < 0.05; n = >6 in each group. (D) Western blot images using protein phosphatase 2A (PP2A) C subunit and phospho‐p38 MAP kinase (Thr180/Tyr182) antibodies to total protein lysate in hippocampal area. The statistical bar charts of internal standardized phosphorylated p38 and PP2A C subunit by β‐actin levels relative to young adult wild‐type C57BL/6J. White and black bars indicate the wild‐type C57BL/6J and Tet‐mev‐1 mice, respectively. All data are expressed as mean ± SD; *P < 0.05; **P < 0.01; n = 4 in each group.