Skip to main content
. 2016 Sep 22;16(1):17–26. doi: 10.1111/acel.12524

Figure 5.

Figure 5

Expression and activation of adenosine monophosphate‐activated protein kinase (AMPK) and cell cycle‐related proteins in aortas of young and old untreated and old rapamycin‐treated mice. (A) Total (open bars) and the ratio of phosphorylated (P‐) to total (hashed bars) AMPK in aortas excised from young and old untreated and rapamycin‐treated mice (N = 5–6/group). Expression of (B) phosphatase and tensin homolog, PTEN, and (C) the cyclin‐dependent kinase inhibitor, p27kip, in aortas excised from young and old untreated and rapamycin‐treated mice (N = 5–6/group). Expression was normalized to GAPDH and blot images are provided in panel (D). * denotes difference from young, and † denotes difference from old. Differences were assessed by one‐way ANOVA with LSD post hoc. Data are means ± SEM, P ≤ 0.05.