Figure 9.

FEL, $F_{}^{\text{SYS}}$, at 300 K for the common binding region (residues 380–386) constructed in 2D PCA space: a) $F_{}^{\text{NonAc}(\mathrm{H}+)}$, b) $F_{}^{\text{Ac}(\mathrm{H}+)}$, c) $F_{}^{\text{NonAc}}$, and d) $F_{}^{\text{Ac}}$. The lowest PMF site, ${\mathit{r}}_{\text{min}}$, is set as $F_{}^{\text{SYS}}({\mathit{r}}_{\text{min}})=0.0$ kcal/mol. Clusters $S_k^{\text{SYS}}$ are shown in FEL. Black filled circle, blue triangle, black filled square, and magenta colored diamond, respectively, denote positions of the bound forms $s_{\mathrm{S}100\mathrm{B}}$, $s_{\text{Sir}2}$, $s_{\text{Cyclin}}$, and $s_{\text{CBP}}$. Table 1 presents the correspondence between the CTD‐fragment type and the binding partner. PCA1 and PCA2 axes are computed from the entire ensemble (i.e., $Q_{\text{sum}}$). Consequently, the PCA axes for all panels are common. [Color figure can be viewed at wileyonlinelibrary.com]