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. 2016 Dec 21;36(2):132–134. doi: 10.15252/embj.201696051

Figure 1. Long‐term expansion and maintenance of murine hair follicle stem cells.

Figure 1

Hair follicle stem cells from the bulge region are characterized by high expression of CD34. Culture of epidermal cell mixtures in 2D conditions in the absence of heterologous cell types leads to an exhaustion of the HFSC pool. Culture of epidermal cell mixtures in 3C conditions leads to the generation of cultures that contain HFSCs and non‐HFSCs (mainly progenitors). 3C conditions allow the expansion of functional HFSCs that generate holoclones upon co‐culturing with dermal cells, generate de novo HFs upon transplantation to mice and share high similarity to FACS‐isolated HFSCs and published HFSC signatures. Moreover, the non‐HFSC population within the 3C culture conditions is able to give rise to the HFSC population.