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. 2017 Jan 11;37(2):383–396. doi: 10.1523/JNEUROSCI.1854-16.2016

Figure 5.

Figure 5.

Spontaneous and evoked vesicles pools are depleted by the elevated spontaneous transmission in cpx−/− and HB mCpx I lines treated with the proton pump blocker Baf. A, Sample spontaneous (left) and evoked (right) traces of the indicated genotypes (Control, cpx−/−, and HB mCpx I). After recording basal minis and EJPs (black traces), larvae were treated with 4 μm Baf for 10 min. After Baf treatment, the recordings were repeated (red lines). Post-treatment depression in both spontaneous and evoked transmission in the mutant lines but not in the control line. B, Spontaneous transmission is significantly reduced in cpx−/− and HB mCpx I lines following Baf treatment (cpx−/− = 52.4 ± 3.8% of basal mini frequency [n = 8] and HB mCpx I = 42.3 ± 3.1% of basal mini frequency [n = 7]). Control animals exhibit only a minor decrease (88.4 ± 6.7% of basal mini frequency [n = 7]). C, cpx−/− and HB mCpx I lines exhibit a modest but significant decrease in evoked transmission following Baf treatment (cpx−/− = 64.8 ± 2.6% of basal EPSP amplitude [n = 8] and HB mCpx I = 68.2 ± 8.2% of basal EPSP amplitude [n = 7]). Control animals exhibited EJP amplitudes of 89.2 ± 5.8% (n = 7) compared with the basal response. Each n value indicates a single muscle recording, with data generated from at least three individual larvae of each genotype. **p < 0.01. ***p < 0.001. ****p < 0.0001.