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. 2017 Jan 18;37(3):637–647. doi: 10.1523/JNEUROSCI.2110-16.2016

Figure 2.

Figure 2.

TREM2 deficiency reduces myeloid cell number. a, Analysis of myeloid cell markers by qPCR revealed no significant changes in expression of the myeloid cell markers PU.1, Tmem119, or CD45 in cortical lysates from 2-month-old APPPS1;Trem2−/− mice compared with APPPS1;Trem2+/+ controls (WT, n = 3 M/3 F; KO, n = 3 M/3 F). However, there was a trend toward reduction in expression levels of PU.1 and Tmem119 in 8-month-old TREM2-deficient mice and a significant reduction in CD45 expression (WT, n = 2 M/2 F; KO, n = 3 M/4 F). b–d, Immunohistochemistry for CD45 at 2 months of age (b; WT, n = 3 M/2 F; KO, n = 3 M/4 F) and 8 months of age (c; WT, n = 4M/2F; KO, n = 4M/2F) revealed a significant decrease in the density of cells expressing high levels of CD45 in the cortex of APPPS1;Trem2−/− mice compared with APPPS1;Trem2+/+ controls (d). ns, Not significant; **p < 0.01; ***p < 0.001.