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. 2017 Jan 18;12(1):e0169504. doi: 10.1371/journal.pone.0169504

Fig 5. mRNA expression within hCSSC and hCF tissue equivalent in comparison to 2D culture.

Fig 5

Changes in keratocyte gene expression within hCSSC and hCF in 3D culture at 9 weeks in comparison to culture on single silk films relative to hCSSC and hCF at day 0. At each time point, RNA was extracted and reverse-transcribed for RT-qPCR. RNA expression of each gene was first normalized against an endogenous reference gene (18S) and then related to the normalized expression level of the target gene at day 0 per each cell type. Keratocan, lumican, PDK4, PTDGS, and ALDH3A1 were significantly up-regulated for 3D hCSSC, suggesting the enhanced keratocytic phenotype in 3D cultures in comparison to 2D culture and hCF. * Significant effect of cell type (p<0.05); * significant effect of culture (p<0.05).