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. 2017 Jan 18;12(1):e0170398. doi: 10.1371/journal.pone.0170398

Fig 2. Effects of pH and temperature on enzyme activity of GusA (red) and GusA-CBD (black).

Fig 2

(A) Temperature: Reactions were performed in 50 mM HEPES buffer (pH 7.5) containing 0.5 mM pNPGA, 0.2 U mL-1 of enzyme for 5 min. (B) pH: The reactions were performed in 50 mM sodium acetate (closed circles) or 50 mM K-phosphate buffer (closed squares) or 50 mM HEPES buffer (closed triangles) containing 0.5 mM pNPGA, 0.2 U mL-1 of enzyme at 60°C for 5 min. Data represent the means of three experiments and error bars represent standard deviation. The relative activities of 100% gusA and gusA-CBD were 102 ± 4.3 and 19 ± 0.6 U mg–1, respectively.