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. 2017 Jan 18;12(1):e0170283. doi: 10.1371/journal.pone.0170283

Fig 3. SrrA binds to DNA fragments immediately preceding the annotated transcriptional start sites for srrA and dps.

Fig 3

Panels A and B; Electromobility gel shift assays (EMSAs) demonstrating binding of SrrA to DNAs that correspond to the 150 base pair segments immediately preceding the annotated transcriptional start sites for the srrA (A) and dps (B). EMSAs were performed with SrrA (15–146 ng) and 8 fM of biotin labeled DNA. For each gel, the samples in lane 1 contain 146 ng SrrA with labeled sample DNA and a 125-fold excess of non-labeled (cold) competitor DNA. The samples in lanes 2–5 contain labeled DNA with varying amounts of SrrA protein (15–146 ng). The samples in lane 6 contain labeled DNA, but no SrrA. The samples in lanes 7–9 show that the interaction of SrrA with DNA is specific. The samples in lane 7 contain 146 ng SrrA with rpsC promoter DNA and a 125-fold excess of non-labeled (cold) competitor DNA. The samples in lanes 8 contain rpsC promoter DNA with 146 ng of SrrA. The samples in lane 9 contain rpsC promoter DNA, but no SrrA.