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. 2017 Jan 18;12(1):e0170347. doi: 10.1371/journal.pone.0170347

Fig 7. LPS/bzATP-activated macrophages and PMA-activated granulocytes produce ROS that kills monocytes in co-culture.

Fig 7

(A) ROS production in LPS/bzATP-activated macrophages. LPS/bzATP was added to the cells at zero time. (B) Apoptosis of monocytes co-cultured for 24 h with LPS/bzATP-activated macrophages. (C) Extracellular ROS produced by granulocytes as a function of time following addition of PMA to the medium. The kinetics were similar to ROS produced by activated monocytes. T cells are shown for comparison; they do not produce ROS. (D) Monocytes were co-cultured with solvent-treated (Granulo-Solvent) or PMA-activated granulocytes (Granulo-PMA) and apoptosis was measured in monocytes after 48 h. Data are the mean of four independent experiments ± SEM, 1-way ANOVA, Tukey’s Multiple Comparison Test, ***p < 0.001.