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. Author manuscript; available in PMC: 2017 Nov 20.
Published in final edited form as: J Biotechnol. 2016 Sep 13;238:9–14. doi: 10.1016/j.jbiotec.2016.09.007

Figure 4. The selection procedure for mutant transgenic hDAT hairy root cultures generated in 100µM MPP+.

Figure 4

Expression of the functional hDAT (transgenic protein-TPG) in hairy root cultures makes them susceptible to toxicity induced by MPP+, which is concentrated intracellularly by the hDAT. Therefore mutants (M) that overproduce inhibitors of the hDAT should have a survival advantage when exposed to MPP+. Although many mutants may survive by other mechanisms, the surviving population should therefore be “enriched” in individual mutants containing metabolites that inhibit the target protein.