Figure 5. Representative transgenic hDAT hairy root cultures after activation tagging mutagenesis (ATM) in medium containing 100 µM MPP+.

This photograph shows about 120 primary hairy roots that have died under selection in 100 µM MPP+ with 2 mutant secondary hairy roots emerging from dead primary hairy roots, and developing normally (2^nd and 3^rd plates, upper row). In the absence of MPP+ about 2,000 secondary hairy roots would have developed from these primary hairy roots after ATM.