Fig 7. The effect of isoflurane on biofilm formation of E. coli K12 parent strain and ion transporter deletion mutants.

The effect of isoflurane on biofilm formed by E. coli K12 strains is shown. Data are shown as mean +/- S.D. of 8 replicates. Statistical analysis was performed using two-way analysis of variance with Bonferroni post hoc analysis for differences between non-exposure and exposure groups. * and ** denote p<0.05 and p<0.01, respectively. For differences between deletion mutants and the K12 parent strain within the non-exposure group, statistical analysis was performed using one-way analysis of variance with Bonferroni post hoc analysis. # and ## denote p<0.05 and p<0.01, respectively.