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Inhibition of growth in the presence of cycloheximide with the antibiotic disc assay. Cells (∼107) from rdnwt or rdn5 strains were spread on YPD plates and grown at 30°C. Two or five microliters of 1 mg/ml cycloheximide were pipetted onto filter paper discs. Again, lighter background color shows suppression by rdn5. Larger clear circles around the rdn5 discs indicate sensitivity towards the antibiotic cycloheximide.
