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Peptidyltransferase activity of rdnwt and rdn5 mutant ribosomes. Double reciprocal plots (1/kobs versus 1/[Puromycin]) for Ac-[3H]Phe-puromycin formation, using complex C prepared with ribosomes from the rdnwt strain (circles) and rdn5 strain (triangles). From these plots, the Ks and kcat values for each strain are determined.
