Table 2. Translational error frequencies in vitro of wild-type and mutant ribosomes in the absence or presence of 50 μM paromomycin (PM).
| Strain | PM (μM) | No. of experiments | Error frequency ± SE |
|---|---|---|---|
| rdnwt | — | 5 | 0.0026 ± 0.0007 |
| 50 | 5 | 0.0079 ± 0.0012 | |
| rdn5 | — | 5 | 0.0087 ± 0.0005a |
| 50 | 5 | 0.0115 ± 0.0004b | |
| rdn5/pS23A-R | — | 5 | 0.0095 ± 0.0005a |
| 50 | 5 | 0.0121 ± 0.0003b | |
| rdn5/pS23A-N | — | 4 | 0.0028 ± 0.0004 |
| 50 | 4 | 0.0044 ± 0.0007 | |
| C/pS23A-wt | — | 4 | 0.0013 ± 0.0005 |
| 50 | 4 | 0.0057 ± 0.0011 | |
| C/pS23A-R | — | 4 | 0.0071 ± 0.0003c |
| 50 | 4 | 0.0170 ± 0.0005d | |
| C/pS23A-N | — | 4 | 0.0010 ± 0.0002 |
| 50 | — | n.d. |
aStatistically different (one-way analysis of variance, F-Scheffè test, p < 0.1) from the rdnwt or rdn5/pS23A-R strains in the absence of PM.
bStatistically different (one-way analysis of variance, F-Scheffè test, p < 0.1) from the rdnwt or rdn5/pS23A-R strains in the presence of 50 μM PM.
cStatistically different (one-way analysis of variance, F-Scheffè test, p < 0.1) from the C/pS23A-wt or C/pS23A-N strains in the absence of PM.
dStatistically different (one-way analysis of variance, F-Scheffè test, p < 0.1) from the C/pS23A-wt strain in the presence of 50 μM PM.