Table 4. Binding capacities of P- and A-sites and peptidyltransferase activity of rdnwt and rdn5 mutant ribosomes.

Strain	P-site binding (% of control)	A-site binding (% of control)	kcat (min−1)	Ks (mM)	kcat/Ks (min−1 mM−1)
rdnwt	100a	100b	1.64 ± 0.11	0.53	3.1
rdn5	136 ± 5c	130 ± 3c	0.92 ± 0.07c	0.55	1.7

^a100% represents 0.180 molecules of Ac-[³H]Phe-tRNA bound to P-site per molecule of ribosome.

^b100% represents 0.126 molecules of [³H]Phe-tRNA bound to A-site per molecule of ribosome.

^cStatistically different (one-way analysis of variance, F-Scheffè test, p < 0.1) from the respective rdnwt.