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. 2004 Oct 11;32(18):5368–5378. doi: 10.1093/nar/gkh877

Figure 3.

Figure 3

RT–PCR confirms the results obtained by Gene Chip analysis. An aliquot of cells that were prepared for microarray analysis was used to isolate total RNA and subsequently to perform the RT–PCR. Genes commonly upregulated by c-Myc in all analyzed cell lines (Ifrd2, Pa2g4, Picalm, Timm10, Lap3, Sms and Gsto1) showed a decreased expression upon doxycycline treatment (2 μg/ml for 12 h), while under the same conditions, expression of Itgam, a gene downregulated by Myc, is increased (A). Expression of the same genes was checked in an additional set of six lymphoma cell lines as biological replicates (B). Examples of up- and downregulated genes specific for B cell lymphoma (Amd1 and CD2) (C) and T cell lymphoma (Thop1 and CD28) (D) are presented. β-actin was used as a housekeeping gene.