Figure 5. MMB triggers a sex- and lifestage-specific attraction in Caenorhabditis species and interferes with mating in C. afra.
(A) Responses of C. elegans hermaphrodites, males, L1 larvae and dauers to a 10−4 dilution of MMB (Mean ± SD, n = 4–10 trials). (B) Responses of Caenorhabditis sp. and P. pacificus to a 10−4 dilution of MMB (Mean ± SD, n = 4–10 trials). (C) Mating behavior of C. afra was recorded. Y axis indicates the time required for the nematodes to mate; if no mating was observed within the 30 min tracking period, data points were represented as 30 min. (D) Chemotaxis of C. afra male and female nematodes in response to 10−2 diacetyl.