Table 2. Cleavage and religation rate constants of the 13 and 18mer Top1–DNA cleavage products trapped by CPT in Control (C) and N²-ethyl-dG adducted (−5) oligonucleotide.

	13mer product		18mer product
	C	−5	C	−5
T1/2 (min)	1.87 ± 0.23	1.88 ± 0.33	0.43 ± 0.1	1.83 ± 0.33
k2 (min−1)	0.37 ± 0.05	0.38 ± 0.06	1.69 ± 0.36	0.38 ± 0.07
k1 (min−1)	0.65 ± 0.23	0.39 ± 0.07	0.1	0.15 ± 0.04
r	0.59 ± 0.1	0.5 ± 0.03	0.06 ± 0.01	0.28 ± 0.02

T_1/2, religation rate half-life determined from the salt reversal experiments (see Figure 3D); k₂, religation rate constant determined from T_1/2; k₁, cleavage rate constant (see Materials and Methods); r, fraction of cleavage product at the plateau (see Figure 3C).

The values are an average of three independent experiments.