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. 2017 Jan 4;6:e22268. doi: 10.7554/eLife.22268

Video 1. Early organization of neuroblasts within the SAG.

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DOI: 10.7554/eLife.22268.006

Tg[cldnb:lynGFP]Tg[Brn3c:GFP] embryos injected with H2B-mCherry mRNA were imaged, and reconstructed cell centers were color-coded according to their location/identity (see legend). The projection view video (large panel) simultaneously displays the topological organization of the cell group selection and tissue architecture as a projection of the GFP channel (plasma membranes in grey) in x,y,z-axes. The distinct visualization modes displayed on the right hand side allow for a detailed 3D-visualization of data during the analyses. Orthogonal views are used to validate cell tracking, the oblique slice view allows orienting the orthoplane along the embryonic axes, and the rendering view permits to display validated cell centers in the context of the whole image volume. ID Dataset: 140423aX.

DOI: http://dx.doi.org/10.7554/eLife.22268.006