Video 6. Spatiotemporal pattern of hair cell generation of sensory maculae in control and MO-neurog1 embryos.
Tg[Brn3c:GFP] embryos were injected with H2B-mCherry mRNA at 1cell-stage (in the presence or absence of MO-neurog1), and differentiated hair cells of the anterior (AM) and posterior (PM) maculae were tracked during 18 hr (Table 1). The centers of hair cells were reconstructed and color-coded according to their time of differentiation as given by the onset of GFP expression. Top row displays reconstructed color-coded cell centers together with imaging data (orthoslice views of the maculae with raw images of hair cells in grey level), middle row shows imaging data alone (GFP signal as volume rendering), and bottom row displays only the reconstructed color-coded cell centers. Volume rendering and reconstructed cell centers panels rotate from dorsal to lateral view to illustrate the 3D-organization of hair cells within the maculae. ID Datasets: 140507aX for control, 140519aX for MO-neurog1.