Figure 1. Maternal inheritance of seed dormancy.

(A) Histograms depicting percent germination of C24, Cvi, C24xCvi F1 and CvixC24 F1 seed populations after-ripened for short (five days), intermediate (25 days) or long (six months) time periods. Germination was scored after 72 hr of seed imbibition (4 replicates, n = 150–200, **p<0.01, ***p<0.001, Student’s t test). Pictures show intermediately after-ripened C24, Cvi, C24xCvi F1 and CvixC24 F1 seeds 72 hr after imbibition (upper panel) and the respective embryos (lower panel) following testa and endosperm removal 4 hr after seed imbibition. (B) Same as (A) using C24^tet, Cvi^tet, C24xCvi^tet F1 and CvixC24^tet F1 seeds. (C) Same as (A) with Col, Cvi, ColxCvi F1 and CvixCol F1 seeds that were not after-ripened (No aft.-rip) or after-ripened for short (10 days), intermediate (20 days) and long (six months) time periods.

DOI: http://dx.doi.org/10.7554/eLife.19573.002

Figure 1—figure supplement 1. Procedure for assessing CvixC24 F1 and C24xCvi F1 hybrid seed production, dry after-ripening and seed germination.

A Cvi mother plant pollinated with C24 pollen produces CvixC24 F1 seeds, whose endosperm cells bear two Cvi maternal genomes and one C24 paternal genome. C24xCvi F1 seeds are obtained by performing the reciprocal cross. CvixC24 and C24xCvi embryo cells bear one Cvi genome and one C24 genome. The testa is composed of dead integumentary maternal tissue, which is homodiploid Cvi (Cvi/Cvi) and C24 (C24/C24) in CvixC24 and C24xCvi seeds, respectively. Freshly harvested F1 hybrid seeds are dry after-ripened for short (five days), intermediate (25 days) or long (six months) time periods. At the end of each after-ripening period, F1 hybrids seeds are imbibed and germination is scored 72 hr after imbibition.

DOI: http://dx.doi.org/10.7554/eLife.19573.003

Figure 1—figure supplement 2. Procedure for the assessment of CvixC24^tet F1 and C24xCvi^tet F1 hybrid seed production, dry after-ripening and seed germination.

Same as Figure 1—figure supplement 1 except that CvixC24^tet and C24^tet are used as pollen donors. The resulting CvixC24^tet F1 hybrid seeds have endosperm cells bearing two Cvi maternal genomes and two C24 paternal genomes, whereas and C24xCvi^tet F1 hybrid seeds have endosperm cells bearing two C24 maternal genomes and two Cvi paternal genomes.

DOI: http://dx.doi.org/10.7554/eLife.19573.004

Figure 1—figure supplement 3. Maternal inheritance of seed dormancy.

Pictures show intermediately after-ripened Col, Cvi, ColxCvi F1 and CvixCol F1 seeds 72 hr after imbibition (upper panel) and the respective embryos (lower panel) following endosperm removal 4 hr after seed imbibition.

DOI: http://dx.doi.org/10.7554/eLife.19573.005