Table 1.

RNA concentrations and read number after RNA-seq using RNA isolated from partially dissected endosperm (with testa still attached to the endosperm), fully dissected endosperm, and testa.

DOI: http://dx.doi.org/10.7554/eLife.19573.006

Sample	RNA concentration ng/µl in 50 µl volume	Total amount of RNA (ng)	Volume of RNA sample used for library preparation (µl)	cDNA library (ng/µl)	Volume of library used for sequencing	Total mapped sequencing reads
Partially dissected endosperm_A	5	250	40	37.4	1.16	39,097,682
Partially dissected endosperm_B	6.4	320	31.25	15.2	2.5	31,930,392
Fully dissected endosperm_A	7	350	28.57	16.4	2.5	39,862,912
Fully dissected endosperm_B	4.4	220	45.45	15.8	2.5	35,254,459
Testa_A	0	0	45.45	0.37	2.5	264,057
Testa_B	0	0	45.45	0.59	2.5	595,710