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. 2004 Oct 8;32(18):e138. doi: 10.1093/nar/gnh134

Figure 3.

Figure 3

(A) Oligonucleotides used for the confirmation of the KpnBI site and (B) determination of the methylated adenines. KpnBI recognition sequences are shown in bold. All the oligonucleotides contain half of the EcoRV sites (ATCGAT) at each end for blunt-end cloning. Other recognition sites are underlined. Note that the B5 and B6 pair contains G/C differences in the sequence, whereas the rest of the oligonucleotide pairs (A1–A2, B1–B2 and B3–B4) contain A/G differences. Plasmids containing these oligonucleotide sequences are shown in parenthesis.