Table 3. KpnBI amino acid sequence homology.
| R-M system | Amino acid length | Identity (%) | Positive (%) | |
|---|---|---|---|---|
| Total | Aligned | |||
| A. Established type I families | ||||
| HsdR (KpnBI) | (1013) | |||
| Type IA EcoKI | 1188 | 641 | 19 | 35 |
| Type IB EcoAI | 813 | NS | — | — |
| Type IC Eco124I | 1033 | 632 | 24 | 42 |
| Type ID StySBLI | 1088 | 790 | 25 | 42 |
| HsdM (KpnBI) | (877) | |||
| Type IA EcoKI | 529 | 390 | 27 | 44 |
| Type IB EcoAI | 489 | 247 | 26 | 40 |
| Type IC Eco124I | 520 | 499 | 31 | 50 |
| Type ID StySBLI | 539 | 493 | 26 | 46 |
| HsdS (KpnBI) | (448) | |||
| Type IA EcoKI | 464 | 444 | 20 | 40 |
| Type IB EcoAI | 589 | 168 | 33 | 56 |
| Type IC Eco124I | 409 | NS | — | — |
| Type ID StySBLI | 401 | 228 | 21 | 42 |
| B. BLAST search to NCBI protein database | ||||
| HsdR (KpnBI) | (1013) | |||
| 1. MmaGORF2294P | 1042 | 1028 | 48 | 65 |
| 2. Hpy99ORF1423P | 991 | 1004 | 47 | 66 |
| 3. HpyAORF1403P | 993 | 1005 | 47 | 65 |
| 4. Tde ATCC35405 | 1039 | 1039 | 46 | 64 |
| 5. XcaCORF2902P | 1096 | 1096 | 28 | 44 |
| HsdM (KpnBI) | (877) | |||
| 1. M.MmaGORF2294P | 808 | 838 | 54 | 68 |
| 2. [Nostoc sp. PCC 7120] | 657 | 684 | 53 | 69 |
| 3. M.HpyAORF1403P | 817 | 835 | 51 | 67 |
| 4. M.Hpy99ORF1423P | 815 | 833 | 51 | 67 |
| 5. M.TdeORFC815P | 871 | 896 | 46 | 61 |
| HsdS (KpnBI) | (448) | |||
| 1. S.SonORF383P | 439 | 201 | 40 | 63 |
| 2. S.MmaGORF2294P | 406 | 404 | 30 | 47 |
| 3. [Nostoc sp. PCC 7120] | 427 | 415 | 29 | 43 |
| 4. S.Mja GI:15669403 | 425 | 417 | 26 | 44 |
| 5. S.Mja GI:2129238 | 425 | 417 | 25 | 44 |
NS, no significant similarity was found.