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. 2017 Jan 18;12(1):e0168233. doi: 10.1371/journal.pone.0168233

Fig 4. Functional aptitude of reovirus activated NK-kB in BrCa.

Fig 4

(A) Reovirus induces NF-kB DNA binding in BrCa cells. BrCa cells were treated with either NV or LV for 12 hours or TNF (20 ng/ml) and nuclear extracts were prepared. 5 micro grams of nuclear proteins was incubated with an IR-dye labeled oligonucleotide consisting of the NF-kB consensus binding sequence and resolved by acrylamide gel electrophoresis and scanned in a LI-COR gel scanner. (B) NF-kB super shift complex is made up of p50 and p65. Nuclear proteins were incubated with 2 μl of super shift antibodies directed against NF-kB p50 or p65 before incubation with the NF-kB p65 probe. The mixtures were resolved in acrylamide gels and scanned as described under Fig 4A.