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MCF7 and HTB 133 cells were transfected with on-target plus siRNA sequences against PUMA or control siRNA and infected with LV. Left panels -Total cellular proteins extracted were subjected to SDS PAGE and blotted against PUMA and actin antibodies. Right panels—HTB 133 and MCF7 cells were transfected with control or PUMA siRNA and infected with LV. Apoptosis was assayed via flow cytometry using annexin V/7AAD, (N = 3, ± SD).
