Fig. 9.

Decreased U-¹³C₆-glucose incorporation into palmitate in pyruvate carboxylase (PC) knockdown cell lines derived from the MDA-MB-231 breast cancer cell line. PC 847 2C and PC 2096 4B cell lines or the shRNA scramble control cell line were maintained in DMEM for 24 h or longer without labeled glucose of glutamine (the same as the zero time control shown in Fig. 3A and B). Cells were then maintained for 18 h in DMEM modified to contain 10 mM [U-¹³C₆]glucose and ¹³C incorporation into palmitate was measured by LC-MS/MS. ^ap < 0.05, ^bp < 0.01 and ^cp < 0.001 vs scramble control.