Table 1. Primers and probe sequences.
| (a) First PCR primer | |
| Ex3-FP | CTGTGCCAATGGGTTTCCA |
| Ex3-RP | CACTGTTTTGGACACATAAGTCTC |
| Ex5-FP | GAAATTTACAAATCTGTGTTCCTGCT |
| Ex5-RP | CATTGGGTCAATAAGGGTTAAGGA |
| Ex6-FP | AGACATGCCAAATGAAACACTCT |
| Ex6-RP | ACTCCTTGGTTTCCTTATTTACAACA |
| Ex7-FP | TTCATAAAGATTGATCAACATGTTCGA |
| Ex7-RP | ACTGGTGCCATGATGACCG |
| Ex8-FP | GAGAGCTGATCTCTATAACTAACCA |
| Ex8-RP | CTCTGATCTTCTGAATGGCGA |
| (b) Second PCR primer | |
| Y61X-CP | TAGGTGGGTATTTTAAGAAAGCTTGT |
| Y61X-SP | GAGAGTTGGGTGCCTCTCTCTTGTACAGGGCGGCCACAAG |
| V200A-CP | GTAGACGTCTTACACACATTCACCAGAGGG |
| V200A-SP | TGGGCATGTTGACATCCTGGCTGAAAAGTACCTCCATTCGGG |
| A221-del-CP | CAGTTGGGCATGTTGACATTTACCCG |
| A221-del-SP | CTATCCGCGTGATTCTTCTAAATAATATTTACCTCCAAGTCCTCTCTCTGC |
| R243C-CP | GCACCTGTAGGCCTTACTTGGATTTTCT |
| R243C-SP | CTCGTGGGAGCACACCCAGATGTGGACCAGCTAGTGAA |
| A261T-CP | CCCACGAGCGCTCCATTCATCTCT |
| A261T-SP | CCTACAGGTGCAGTAGAGCCCTTTCTCAAAGGCTTCCTTGG |
| A334T-CP | CCTCCCCAACAGTCTTCCATTACCAAGTAAAG |
| A334T-SP | CCTTTGAGATTTCTCTGGGATGTTCTCACTCTCGGCCACGGTGCCAT |
| W382X-CP | AGACCTACTCCTTCCTAATTTACACAGAGGTAG |
| W382X-SP | AAGAGTGATTCATACTTTCTGCTCCACCAGTCTGACCAGC |
| (c) Probe | |
| Y61X-W probe | AAAGCTTGTGTCATCATCTTCAGGTAACAGGAATGTAT |
| Y61X-M probe | AAAGCTTGTGTCATCATCTTCAGGTAACAGGAATGTAA |
| V200A-Wprobe | GGGTCCCCTGGTCGAAGCATTGGAATCCAGAAAC-CAGT |
| V200A-Mprobe | GGGTCCCCTGGTCGAAGCATTGGAATCCAGAAAC-CAGC |
| A221-del-Wprobe | TGGAGGTACTTTTCAGCCAGGATGTAACATTGGA-GAAG |
| A221-del-Mprobe | ATGGAGGTACTTTTCAGCCAGGATGTAACATTGG-AGAA |
| R243C-Wprobe | TCATTCAACAGAGAGTCGATGAAGAGATGAATGG-AGCG |
| R243C-Mprobe | TCATTCAACAGAGAGTCGATGAAGAGATGAATG-GAGCA |
| A261T-Wprobe | CATCGACTCTCTGTTGAATGAAGAAAATCCAAGT-AAGG |
| A261T-Wprobe | CATCGACTCTCTGTTGAATGAAGAAAATCCAAGT-AAGA |
| A334T-Wprobe | TTTTTCTGGGACTGAGAGTGAAACCCATACCAAT-CAGG |
| A334T-Mprobe | TTTTTCTGGGACTGAGAGTGAAACCCATACCAAT-CAGA |
| W382X-Wprobe | TAGATATTGGAGAACTACTCATGTTGAAGCTCAA-ATGG |
| W382X-Wprobe | TAGATATTGGAGAACTACTCATGTTGAAGCTCAA-ATGA |
In (a), FP denotes ‘forward primer’ and RP denotes ‘reverse primer’. In (b), CP and SP indicate ‘counter primer’ and ‘special primer’, respectively. The underlined nucleotides in the SP are the tag sequences essential to make a stem structure in a self-loop formation (cf. Figure 3D). In (c), W probe and M probe indicate ‘wild-type probe’ and ‘mutant-type probe’, respectively.