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Release of Shh from permeabilized resting rabbit gastric glands in response to increased salt concentration. Resting rabbit gastric glands were permeabilized with 20 μg/ml digitonin in isotonic sucrose, incubated with 0, 50, 100, or 150 mM KCl for 5 min. Pellet (glands) and supernatant proteins (10 μg) were separated on a 4–20% SDS gradient gel and immunoblotted for Shh and H+-K+-ATPase. Immunoblots for time 0 (control, A) and 5 min after digitonin incubation (B) are shown.
