Fig. 9.

Expression of Shh at the basolateral membrane of gastric parietal cells. Rabbit gastric glands were immunostained for Shh (red, A) and the basolateral membrane marker, γ−actin (green, B). The merged image is shown in C, together with optical sections spaced 0.5 μm in the z-axis of glands (x-z and y-z). This image was taken at 6.50 μm. The green line in the x-y image is the location of the x-z slice. The red line in the x-y image is the location of the y-z slice. Colocalization is indicated by the yellow color. D: Shh immunofluorescence in the gastric gland from A at 3 different focal planes (2.50, 6.50, 12.00 μm). A series of 0.5-μm optical sections in the z-axis of the gland are shown. BL, basolateral membrane; CM, canalicular membrane.