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The number of calcified nodules formed by valvular interstitial cells (VICs) cultured on different substrate coatings for 5 days; nodules were identified and counted following Alizarin Red S staining. Coll, collagen type I; FN, fibronectin; FB, fibrin; TCPS, tissue culture polystyrene (uncoated control). Values are means ± SD. *P < 0.0001 compared with Coll or FN.
