Fig. 1.

A: experimental design used in the present study. Arm-A: serum starvation (medium only) was applied at time 0 in cell cultures. Prucalopride (1 nM) was applied 30 min after challenge with H₂O₂ (200 μM). Arm-B: the 5-HT₄ antagonist GR113808 (10 nM) was applied 30 min before either prucalopride or H₂O₂. B: Western blot of total cell lysates with antibody to 5-HT₄ protein and with anti-vinculin in SH-SY5Y (lane 1), SK-N-BE (lane 2), SK-N-SH (lane 3), LAN-1 (lane 4), Neuro2A (N2A, lane 5), and HEK293 (lane 6). C: separate Western blot of total cell lysates with antibody to 5-HT₄ protein and with anti-vinculin in SH-SY5Y (lane 1) and human enteric neurospheres (lane 2). D: total cell lysates prepared from SH-SY5Y (a) and human enteric neurospheres (b) immunoblotted with anti-HuC/D, anti-p75, and anti-vinculin antibodies.